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Br J Ophthalmol 2005;89:1223-1224 doi:10.1136/bjo.2005.067991
  • Letter

Nylon paper: an alternative to cellulose acetate paper for use in conjunctival impression cytology

  1. M K B Meena1,
  2. A Khuteta1,
  3. H Saxena2
  1. 1Department of Ophthalmology, SMS Hospital, Jaipur, India
  2. 2Department of Pathology, SMS Hospital, Jaipur, India
  1. Correspondence to: Dr Monisha K Brijlal Meena Department of Ophthalmology, SMS Hospital, Jaipur, India, dr_ophthalyahoo.co.in
  • Accepted 5 February 2005

Conjunctival imprint cytology (CIC) offers valuable clues to the diagnosis and study of the pathogenesis of conjunctival disorders.1–3 The technique involves the use of a membrane filter paper to pick up a layer of cells from the conjunctival surface.

This study was conducted to evaluate the results of CIC using a nylon filter paper compared to routinely used cellulose acetate paper. It involved 20 normal asymptomatic eyes of 10 participants. The participants had no ocular complaints and they were evaluated to rule out any conjunctival disease.

The procedure was explained to the participants and their consent was given.

CIC was done to assess the normal conjunctival cytology using Ultipor (nylon6, 6) and sartorius-type 111 (cellulose acetate paper).

The physical properties such as pore size and thickness of the two papers were matched.

Technique

Cellulose acetate and nylon membrane filters were cut into small triangles and squares respectively to make their identification easy after staining. The conjunctiva was anaesthetised by topical 4% …

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