rss
Br J Ophthalmol 2006;90:96-98 doi:10.1136/bjo.2005.078394
  • Clinical science
    • Extended reports

Expression of glutamine synthetase and cell proliferation in human idiopathic epiretinal membrane

  1. S Kase1,
  2. W Saito1,
  3. M Yokoi2,
  4. K Yoshida1,
  5. N Furudate1,
  6. M Muramatsu1,
  7. A Saito1,
  8. M Kase2,
  9. S Ohno1
  1. 1Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan
  2. 2Department of Ophthalmology, Teine Keijin-kai Hospital, Sapporo, Japan
  1. Correspondence to: Satoru Kase MD, PhD, Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638 Japan; kaseron{at}med.hokudai.ac.jp
  • Accepted 29 August 2005

Abstract

Background/aim: The mechanisms of the cellular origin and cell proliferation in the idiopathic epiretinal membrane (ERM) are unsolved. The aim of this study was to examine the expression of cell cycle related molecules and glutamine synthetase (GS), which is expressed in Müller cells and their processes, in ERM tissues.

Methods: The ERMs were surgically removed using pars plana vitrectomy. Formalin fixed, paraffin embedded ERM tissues were analysed by immunohistochemistry with anti-cyclin D1, p27 (KIP1), proliferating cell nuclear antigen (PCNA), and GS antibodies.

Results: The histopathological findings showed that all the ERMs consisted of oval or spindle mononuclear cells with thin collagen-like tissues. Immunoreactivity for GS was detected in collagen-like tissues of ERM, presenting a continuous, isodense pattern. GS immunopositive cells in all cases expressed PCNA in their nuclei. Nuclear immunoreactivity for cyclin D1 was noted in the ERM constituent cells, whereas p27 (KIP1) positive nuclei were not detected.

Conclusion: Cyclin D1 and PCNA were expressed in the idiopathic ERM, which was mainly derived from Müller cells and extensions of their processes.

Footnotes

    This Article

    1. Abstract
    2. Full text
    3. PDF

    Services

    1. Email this link to a friend
    2. Alert me when this article is cited
    3. Alert me if a correction is posted
    4. Alert me when eletters are published
    5. Article Usage Statistics
    6. Similar articles in this journal
    7. Similar articles in Web of Science
    8. Similar articles in PubMed
    9. Add article to my folders
    10. Download to citation manager
    11. Request permissions

    Responses

    1. Submit a response
    2. No responses published

    Social bookmarking

    Register for free content


    Free sample
     This recent issue is free to all users to allow everyone the opportunity to see the full scope and typical content of BJO.
    View free sample issue >>

    Free archive
    The full back archive is now available for BJO. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006, back to volume 1 issue 1.
    Register to access the free archive >>

    Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.