Article Text

PDF
Trefoil factor family 1, MUC5AC and human leucocyte antigen-DR expression by conjunctival cells in patients with glaucoma treated with chronic drugs: could these markers predict the success of glaucoma surgery?
  1. M Souchier1,
  2. N Buron2,
  3. P O Lafontaine1,
  4. A M Bron1,
  5. C Baudouin3,
  6. C Creuzot-Garcher1
  1. 1Department of Ophthalmology, University Hospital, Dijon, France
  2. 2INSERM U 517, University Hospital
  3. 3Centre Hospitalier National d’Ophtalmologie des Quinze Vingts, Paris, France
  1. Correspondence to: A M Bron Service d’Ophtalmologie, Hôpital Général, 21000 Dijon, France;alain.bron{at}chu-dijon.fr

Abstract

Aims: To evaluate conjunctival expression of trefoil factor family (TFF)1, MUC5AC and human leucocyte antigen (HLA)-DR in patients with glaucoma treated with topical drugs, and to determine whether these parameters can predict the outcome of glaucoma surgery.

Methods: 77 conjunctival impression cytology specimens were collected from 77 patients with glaucoma (66 receiving drops with preservative and 11 treated with preservative-free drops) and 43 controls. TFF1, MUC5AC and HLA-DR expression was analysed using flow cytometry. Trabeculectomy was performed in 56 patients; success was defined as an intraocular pressure (IOP) ⩽15 mm Hg without any IOP-lowering drug at 6 months.

Results: The expression of TFF1, MUC5AC and HLA-DR was significantly higher in patients than in controls (p = 0.01, 0.05 and 0.004, respectively). A higher expression of MUC5AC was found in patients treated with preserved drops than in those receiving unpreserved drops (p = 0.04). A higher MUC5AC expression and a lower HLA-DR expression was observed in successful glaucoma surgeries than in failures.

Conclusions: TFF1 and MUC5AC secretions are probably a response to mild ocular surface changes caused by long-term use of topical treatment. Their increased expression could be a predicting factor of further successful glaucoma surgery.

  • HLA, human leucocyte antigen
  • IOP, intraocular pressure
  • PBS, phosphate-buffered saline
  • TTF, trefoil factor family

Statistics from Altmetric.com

Footnotes

  • Published Online First 29 June 2006

  • Competing interests: None.

  • This work has been partly presented as a poster at the ARVO meeting, 2005.

    Ethical approval: The protocol of this prospective, observational, case–control study was approved by the local ethics committee of the Burgundy region, located in Dijon, France.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.