Article Text

PDF
Capacity of ocular infiltrating T helper type 1 cells of patients with non-infectious uveitis to produce chemokines
  1. H Takase1,
  2. S Sugita1,
  3. C Taguchi2,
  4. Y Imai1,
  5. M Mochizuki1
  1. 1Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University Graduate School, Tokyo, Japan
  2. 2Department of Ophthalmology, Kurume University School of Medicine, Fukuoka, Japan
  1. Correspondence to: Hiroshi Takase MD, PhD, Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University Graduate School, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan; h.takase.oph{at}tmd.ac.jp

Abstract

Background/aims: Chemokines are key molecules that initiate leucocyte infiltration to the inflammatory site. The involvement of chemokines in uveitis is well studied, yet the source of this molecule in the inflamed eye is not clearly identified. The possible sources of chemokines are ocular resident cells or the inflammatory cells infiltrated to the eye. Here the authors examined whether ocular infiltrating T cells of uveitis patients do produce chemokines.

Methods: T cell clones (TCCs) were established from ocular infiltrating cells of patients with non-infectious uveitis. TCCs were characterised using flow cytometry. Spontaneous production of chemokines by TCCs was evaluated by ELISA.

Results: TCCs from ocular infiltrating cells were revealed to be memory activated Th1 type CD4 positive cells. Those TCCs produced larger amounts of chemokines than TCCs from peripheral blood mononuclear cells of uveitis or healthy donors.

Conclusions: The present data indicate that ocular infiltrating T cells of patients with non-infectious uveitis produce chemokines and recruit further infiltrating lymphoid cells. Such T cells may have roles in the prolonged/chronic state of non-infectious uveitis.

  • AAU, acute anterior uveitis
  • AqH, aqueous humour
  • EAU, experimental autoimmune uveitis
  • IL, interleukin
  • IP, interferon inducible protein
  • MIP, macrophage inflammatory protein
  • PBMC, peripheral blood mononuclear cells
  • PBS, phosphate buffered saline
  • PE, phycoerythrin
  • RANTES, regulated on activation, normal T express and secreted
  • TCCs, T cell clones
  • Th 1 cells, T helper type 1 cells
  • VKH, Vogt-Koyanagi-Harada
  • chemokine
  • uveitis
  • T cell
  • aqueous humour
  • AAU, acute anterior uveitis
  • AqH, aqueous humour
  • EAU, experimental autoimmune uveitis
  • IL, interleukin
  • IP, interferon inducible protein
  • MIP, macrophage inflammatory protein
  • PBMC, peripheral blood mononuclear cells
  • PBS, phosphate buffered saline
  • PE, phycoerythrin
  • RANTES, regulated on activation, normal T express and secreted
  • TCCs, T cell clones
  • Th 1 cells, T helper type 1 cells
  • VKH, Vogt-Koyanagi-Harada
  • chemokine
  • uveitis
  • T cell
  • aqueous humour

Statistics from Altmetric.com

Footnotes

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.