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Pars planitis is associated with an increased frequency of effector-memory CD57+ T cells
  1. Miguel Pedroza-Seres1,
  2. Marisela Linares1,
  3. Stephanie Voorduin1,
  4. Rojas-Ramos Enrique2,
  5. Ricardo Lascurain3,
  6. Yonathan Garfias1,
  7. Maria Carmen Jimenez-Martinez1
  1. 1
    Research Unit and Uvea Department, Institute of Ophthalmology “Fundación Conde de Valenciana” Mexico DF, Mexico
  2. 2
    Departamento de Inmunología Clínica y Alergia, Hospital 1o de Octubre, ISSSTE, México DF, Mexico
  3. 3
    Departamento Bioquímica, Fac Med UNAM, Mexico DF, Mexico
  1. Dr M C Jimenez-Martinez, Research Unit, Institute of Ophthalmology, “Fundación Conde de Valenciana”, Chimalpopoca 14, Col Obrera, CP 06800, México DF, México; mcjimenezm{at}investigacioniocv.com

Abstract

Aim: To evaluate the frequency, phenotype and the potential function of CD57+ T cell subsets in patients with pars planitis.

Methods: CD4+CD57+ and CD8+CD57+ T cells were quantitated in peripheral blood from 15 patients with pars planitis and 15 healthy controls. To evaluate the phenotype and potential function of CD57+ T cell subsets CCR7, CD27, CD28, CD45RA, CD45RO, intracellular IFN-γ, IL-4, perforin and granzyme-A expression were assessed by flow cytometry.

Results: CD57+ T cells subsets were increased in patients with pars planitis (p = 0.002). The majority of CD4+CD57+ T cells were CCR7−CD27−CD28−CD45RO+, while the most CD8+CD57+ T cells were CCR7−CD27−CD28−CD45RA+. The number of cells positive for intracellular IFN-γ and IL-4 was higher in the CD57+ T cell populations. A greater number of CD8+CD57+ T cells than CD8+CD57− T cells were positive to perforin (p = 0.006) and granzyme-A (p = 0.01).

Conclusions: CD57+ T cells had a phenotype associated with peripheral memory (CCR7−CD27−CD28−). Cytokine production by CD57+ T cells suggests that these cells may play a role in helper cell regulation. High expression of intracellular proteins involved in cytotoxicity suggests that CD8+CD57+ T cells may play an effector role. Taken together, this study proposes that CD57+ T cells function as memory-effector T cell subsets during pars planitis pathogenesis.

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Footnotes

  • Funding: This work was supported by a grant from National Council of Science and Technology (CONACYT-SALUD 2005-14108).

  • Competing interests: None.

  • Abbreviations:
    PBMC

    peripheral blood mononuclear cell

    PBS

    phosphate buffered saline

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