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The role of clusterin in retinal development and free radical damage
  1. Jeong Hun Kim1,*,
  2. Jin Hyoung Kim2,*,
  3. Young Suk Yu1,
  4. Bon-Hong Min3,
  5. Kyu-Won Kim2
  1. 1
    Department of Ophthalmology, Seoul National University College of Medicine & Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea
  2. 2
    NeuroVascular Coordination Research Center, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea
  3. 3
    Department of Pharmacology & BK21 Program for Medical Sciences, College of Medicine, Korea University, Seoul, Korea
  1. Professor Y S Yu, Department of Ophthalmology, Seoul National University College of Medicine & Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea; ysyu{at}snu.ac.kr

Abstract

Aim: To assess the role of clusterin in retinal vascular development and in free radical damage in vivo and in vitro.

Methods: The expression of clusterin, von Willebrand factor (vWF), flk-1, heat shock protein 27 (Hsp27) and heat shock protein 70 (Hsp70) was examined in the retinas of developing mice and oxygen-induced retinopathy (OIR) mice by immunofluorescence staining and western blot analysis. Hydrogen peroxide (H2O2)-pretreated human retinal endothelial cells (HREC) and astrocytes were cultured in the presence or absence of exogenous clusterin, and then the cell viability was measured using the MTT assay and DAPI staining.

Results: Clusterin was expressed mainly in the inner retina and co-localised with vWF, an endothelial cell marker. During the mouse developmental process, clusterin expression was decreased, which was similar to the expression of flk-1, vWF and Hsp27. Furthermore, in the OIR model, clusterin expression changed in a similar way to both vWF and Hsp27. Under hypoxic conditions, clusterin expression increased in HREC and astrocytes. In H2O2-pretreated HREC and astrocytes, clusterin protected against apoptotic cell death.

Conclusions: These results suggest that clusterin is associated with protection from apoptotic retinal cell death in retinal development and in free radical damage.

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Footnotes

  • Competing interests: None declared.

  • *These authors contributed equally to this work.

  • Abbreviations:
    GCL

    ganglion cell layer

    HREC

    human retinal endothelial cells

    Hsp

    heat shock protein

    INL

    inner nuclear layer

    IPL

    inner plexiform layer

    OIR

    oxygen-induced retinopathy

    vWF

    von Willebrand factor

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