Identification of ganglion cell neurites in human subretinal and epiretinal membranes
- Geoffrey P Lewis1,
- Kellen E Betts1,
- Charanjit S Sethi3,
- David G Charteris3,
- Sarit Y Lesnik-Oberstein4,
- Robert L Avery1,
- Steven K Fisher1,2
- 1Neuroscience Research Institute, University of California, Santa Barbara, California, USA
- 2Department of MCD Biology, University of California, Santa Barbara, California, USA
- 3Moorfields Eye Hospital, London, UK
- 4The Academic Medical Center Amsterdam, University of Amsterdam, Amsterdam, The Netherlands
- G P Lewis, Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA; g_lewis{at}lifesci.ucsb.edu
- Accepted 1 November 2006
- Published Online First 15 November 2006
Abstract
Aim: To determine whether neural elements are present in subretinal and epiretinal proliferative vitreoretinopathy (PVR) membranes as well as in diabetic, fibrovascular membranes removed from patients during vitrectomy surgery.
Methods: Human subretinal and epiretinal membranes of varying durations were immunolabelled with different combinations of antibodies to glial fibrillary acidic protein, vimentin, neurofilament protein and laminin.
Results: Anti-neurofilament-labelled neurites from presumptive ganglion cells were frequently found in epiretinal membranes and occasionally found in subretinal membranes. In addition, the neurites were only observed in regions that also contained glial processes.
Conclusions: These data demonstrate that neuronal processes are commonly found in human peri-retinal cellular membranes similar to that demonstrated in animal models. These data also suggest that glial cells growing out of the neural retina form a permissive substrate for neurite growth and thus may hold clues to factors that support this growth.
Footnotes
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Competing interests: None declared.
- Abbreviations:
- CNS
-
central nervous system
- GFAP
-
glial fibrillary acidic protein
- PVR
-
proliferative vitreoretinopathy
- RPE
-
retinal pigment epithelium
