Table 3 Oligoprobe sequences and conditions for methylation-sensitive dot-blot assay
| Gene | CG-probe sequences (5′–3′) | TG-probe sequences (5′–3′) | Tm Hyb(°C) | Tm washes(°C) |
| p16 | TCGGAGGGGGTTTTTTCGTT | GTTGGAGGGGGTTTTTTTGTT | 50 | 53 |
| TIMP3 | GTCGATGAGGTAATGCGGTT | GGTTGATGAGGTAATGTGGTT | 50 | 52 |
| RASSF1 | CAAAACCAACGAAACACGAAC | CAAAACCAACAAAACACAAACC | 50 | 53 |
| RARB | GGAAAGAAAACGTCGGTTTGT | GGAAAGAAAATGTTGGTTTGTG | 50 | 53 |
| hTERT | TAGTTGCGTTGTCGGGGTTA | GTAGTTGTGTTGTTGGGGTTA | 50 | 53 |
| FHIT | GGTTTCGTTTTTATCGTGGG | GGGTTTTGTTTTTATTGTGGG | 48 | 52.5 |
| APC | GATGCGGATTAGGGCGTTTT | GGATGTGGATTAGGGTGTTTT | 50 | 53 |
