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Br J Ophthalmol 92:1275-1282 doi:10.1136/bjo.2008.138768
  • Original Article
    • Laboratory science

Conjunctival and corneal reactions in rabbits following short- and repeated exposure to preservative-free tafluprost, commercially available latanoprost and 0.02% benzalkonium chloride

Open Access
  1. H Liang1,2,
  2. C Baudouin2,3,4,
  3. A Pauly1,2,
  4. F Brignole-Baudouin1,2,3
  1. 1
    Department of Toxicology, Faculty of Biological and Pharmacological Sciences, University Paris Descartes, Paris, France
  2. 2
    INSERM UMR S 592, Institute of Vision, University of Paris 6, Paris, France
  3. 3
    Department of Ophthalmology III, Quinze-Vingts National Ophthalmology Hospital, Paris, France
  4. 4
    Ambroise Paré Hospital, APHP, University of Versailles, Paris, France
  1. Dr F Brignole-Baudouin, INSERM UMR S 592, Institute of Vision, University of Paris 6, Paris, France, 17, rue Moreau, 75012, Paris, France; frbaudouin{at}aol.com
  • Accepted 25 June 2008

Abstract

Aim: To compare the conjunctival and corneal reactions of commercially available solution of latanoprost (Xalatan) and preservative-free (PF) tafluprost in rabbits.

Methods: The rabbits received 50 μl of phosphate-buffered saline (PBS), PF-tafluprost 0.0015%, latanoprost 0.005% or benzalkonium chloride (BAK) 0.02%; all solutions were applied at 5 min intervals for a total of 15 times. The ocular surface toxicity was investigated using slit-lamp biomicroscopy examination, flow cytometry (FCM) and on imprints for CD45 and tumour necrosis factor-receptor 1 (TNFR1) conjunctival impression cytology (CIC) and corneal in vivo confocal microscopy (IVCM). Standard immunohistology also assessed inflammatory/apoptotic cells.

Results: Clinical observation and IVCM images showed the highest ocular surface toxicity with latanoprost and BAK, while PF-tafluprost and PBS eyes presented almost normal corneoconjunctival aspects. FCM showed a higher expression of CD45+ and TNFR1+ in latanoprost- or BAK-instilled groups, compared with PF-tafluprost and PBS groups. Latanoprost induced fewer positive cells for inflammatory marker expressions in CIC specimens compared with BAK-alone, both of which were higher than with PF-tafluprost or PBS. Immunohistology showed the same tendency of toxic ranking.

Conclusion: The authors confirm that rabbit corneoconjunctival surfaces presented a better tolerance when treated with PF-tafluprost compared with commercially available latanoprost or BAK solution.

Footnotes

  • Funding: This report was supported by unrestricted grants from the Department of Toxicology, Faculty of Biological and Pharmacological Sciences, INSERM UMR S 592, and Santen Oy, Tampere, Finland.

  • Competing interests: None.