Article Text

PDF
Implications of bevacizumab on vascular endothelial growth factor and endostatin in human choroidal neovascularisation
  1. O Tatar1,
  2. K Shinoda2,
  3. E Kaiserling3,
  4. C Claes4,
  5. C Eckardt5,
  6. T Eckert5,
  7. G Pertile6,
  8. V Boeyden4,
  9. G B Scharioth7,
  10. E Yoeruek1,
  11. P Szurman1,
  12. K U Bartz-Schmidt1,
  13. Tuebingen Bevacizumab Study Group1,
  14. S Grisanti8
  1. 1
    University Eye Clinic at the Centre for Ophthalmology of the Eberhard-Karls-University, Tuebingen, Germany
  2. 2
    Laboratory of Visual Physiology, National Institute of Sensory Organs, Tokyo, Japan
  3. 3
    Department of Pathology, Eberhard-Karls University, Tuebingen, Germany
  4. 4
    AZ- Sint Augustinus Hospital, Department Achtersegment, Antwerp, Belgium
  5. 5
    Augenklinik der Staedtischen Kliniken, Frankfurt am Main, Germany
  6. 6
    Department of Ophthalmology, Sacro Cuore Hospital, Negrar, Italy
  7. 7
    Augenzentrum Recklinghausen, Germany
  8. 8
    Department of Ophthalmology at the University of Luebeck, Luebeck, Germany
  1. Professor S Grisanti, Department of Ophthalmology at the University of Luebeck, Luebeck Germany, Ratzeburger Allee 160, 23538 Luebeck, Germany; salvatore.grisanti{at}uk-sh.de

Abstract

Aim: To evaluate the implications of intravitreal bevacizumab on proangiogenic vascular endothelial growth factor (VEGF) with regard to the endogenous angiogenesis inhibitor endostatin in human choroidal neovascularisation (CNV) secondary to age-related macular degeneration.

Methods: Retrospective review of an interventional case series of 48 patients who underwent full macular translocation surgery with removal of CNV. Twenty-five patients were treated with intravitreal bevacizumab injection 1 to 154 days prior to surgery (bevacizumab CNV). Twenty-three CNV without any kind of previous treatment were used as controls (control CNV). CNV were stained for CD34, cytokeratin18, VEGF, endostatin and E-selectin. A “predominance score of VEGF over endostatin” (PS) was defined by the difference between VEGF and endostatin staining scores.

Results: Bevacizumab CNV revealed a weaker VEGF expression in endothelial cells (p = 0.0245) but significantly more intense endostatin in retina pigment epithelium (RPE) (p = 0.0001) and stroma (p<0.0001). Consequently, PS was significantly lower in RPE (p = 0.02), vessels (p = 0.03) and stroma (p = 0.0004) in bevacizumab CNV. The intensity of E-selectin expression in bevacizumab CNV was comparable with that in control CNV.

Conclusions: A shift within the angiogenic balance in terms of decreased VEGF predominance over endostatin is detected in human CNV treated with bevacizumab.

Statistics from Altmetric.com

Footnotes

  • Funding: Grant Support: Vision 100 Foundation and Jung Foundation.

  • Competing interests: None.

  • Ethics approval: Ethics approval was provided by the Ethics Committee of the University of Tuebingen.

  • Patient consent: Obtained.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.