Article Text

PDF
Experimental microablation of choroidal tissue for autologous retinal pigmentepithelium–choroid translocation with the pulsed electron avalanche knife (PEAK-fc)
  1. Felix Treumer,
  2. Benjamin Sattler,
  3. Arnd Bunse,
  4. Alexa Klettner,
  5. Jost Hillenkamp,
  6. Johann Roider
  1. Department of Ophthalmology, University Medical Center Schleswig-Holstein, Campus Kiel, Germany
  1. Correspondence to Dr Felix Treumer, Department of Ophthalmology, University Medical Center Schleswig-Holstein, Arnold-Heller Str. 3, Haus 25, D-24105 Kiel, Germany; ftreumer{at}auge.uni-kiel.de

Abstract

Aim To test the microablation of excess graft choroidal tissue with the pulsed electron avalanche knife (PEAK-fc) in an in-vitro model of autologous retinal pigment epithelium (RPE)–choroid translocation.

Methods Choroidal tissue of porcine RPE–choroid explants was ablated with the PEAK-fc. Tissue morphology was assessed by light microscopy (LM) and scanning electron microscopy (SEM). The amount of ablated choroidal tissue was analysed as a function of three PEAK-fc parameters: (1) amplitude of biphasic voltage (70–100%); (2) distance between choroidal tissue and tip of the PEAK-fc (0–300 μm); and (3) exposure time (2–8 s).

Results LM and SEM showed a smooth plain within the ablation area with well defined cutting edges and preserved adjacent tissue structure. The mean amount of ablated tissue correlated linearly with applied voltage (range 79–120 μm, r=0.34) and distance between choroidal tissue and PEAK-fc tip (range 10–100 μm, r=0.74). The mean amount of ablated tissue increased with exposure time between 2 and 4 s (36–88 μm, r=0.4) and remained constant between 4 and 8 s.

Conclusion The PEAK-fc accurately microablates choroidal tissue in-vitro. The adjacent choroidal tissue structure and Bruch's membrane are preserved. Patient studies are required to test the PEAK-fc in RPE–choroid translocation surgery.

  • RPE transplantation
  • pulsed electron avalanche knife PEAK-fc
  • choroidal ablation
  • AMD
  • choroid
  • retina
  • macula
  • degeneration
  • treatment surgery

Statistics from Altmetric.com

Footnotes

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.