Article Text

PDF
In vivo confocal microscopy of conjunctival goblet cells in patients with Sjögren's syndrome dry eye
  1. Jiaxu Hong,
  2. Wenqing Zhu,
  3. Hong Zhuang,
  4. Jianjiang Xu,
  5. Xinghuai Sun,
  6. Qihua Le,
  7. Gang Li,
  8. Yan Wang
  1. Department of Ophthalmology, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, Shanghai, PR China
  1. Correspondence to Dr Jianjiang Xu, Department of Ophthalmology, Eye & ENT Hospital, School of Shanghai Medicine, Fudan University, 83 Fenyang Road, Shanghai 200031, PR China; jianjiangxu{at}126.com

Abstract

Background To study the morphology and the density of conjunctival goblet cells (GC) in patients with Sjögren's syndrome dry eye with in vivo laser scanning confocal microscopy (LSCM), and to explore its correlation with the GC density detected by impression cytology.

Methods A total of 43 Sjögren's syndrome dry eye patients were recruited. All were required to fill in the Ocular Surface Disease Index Questionnaires. The tear break-up time was measured, followed by corneal fluorescein staining examination and Schirmer I test. The images of conjunctiva were taken by the Heidelberg retina tomography (HRT-II)/Rostock cornea module. Finally, the specimens for impression cytology were obtained. SPSS V.13.0 software was used to analyse the data.

Results Tear film function test showed that all patients had moderate to severe dry eye. The GC in LSCM images was characterised as a large hyper-reflective oval-shape cell with relatively homogeneous brightness. Though GC density assessed by LSCM (332±137) cells/mm2 was higher than that measured by impression cytology (200±141) cells/mm2, they showed a significant positive correlation, ρ=0.908 (p<0.05).

Conclusion Conjunctival GC could be easily discriminated under the LSCM. LSCM may be a valuable tool in monitoring the progress and the follow-up of patients with Sjögren's syndrome dry eye.

  • Conjunctiva
  • imaging
  • pathology
  • diagnostic tests/investigation

Statistics from Altmetric.com

Footnotes

  • Funding This work was supported partly by grants from the Key Clinic Medicine Research Program, the Ministry of Health, China (2007-2009).Other funders: the Ministry of Health, China.

  • Competing interests None.

  • Patient consent Obtained.

  • Ethics approval This study was conducted with the approval of the Eye & ENT Hospital, Shanghai, China.

  • Provenance and peer review Not commissioned; externally peer reviewed.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles