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Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y
  1. S G Shah1,
  2. S Sharma2,
  3. M Fernandes1,
  4. M Lakshmipathy1
  1. 1Cornea and Anterior Segment Services, L V Prasad Eye Institute, GMR Varalakshmi Campus, Vishakapatnam, Andhra Pradesh, India
  2. 2Ocular Microbiology Service, Hyderabad Eye Research Foundation, L V Prasad Eye Institute, Bhubaneswar, Orissa, India
  1. Correspondence to Dr Merle Fernandes, Cornea and Anterior Segment Services, L V Prasad Eye Institute, GMR Varalakshmi Campus, Visakhapatnam, Plot 87/2, Hanumanthawaka Junction, Visakhapatnam, Anhdra Pradesh 530040, India; merle{at}lvpei.org

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We read with great interest the article by Shiraishi et al1 who have described a good technique for the laboratory diagnosis of Acanthamoeba cysts in corneal scrapings of patients with clinical features of Acanthamoeba keratitis using Fungiflora Y (FFY). We would like to share some of our comments related to this study.

Though described by the authors as a simple and fast technique, it calls for the use of expensive machinery such as the cryotome and expertise to take frozen sections. The availability of such equipment cannot be taken for granted even …

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