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We appreciate and the letter by Dr Shah et al regarding our article. They highlighted the usefulness of calcoflour white staining (CFW) and recommended that comparative trials between Fungiflora Y staining (FFY) and CFW be performed. As we have reported, we acknowledge the usefulness of CFW for detecting acanthamoeba cysts and agree on the need of a comparative study. The reason why we use FFY for detecting acanthamoeba cysts is that this dye is readily available in Japan, and the cost is about 1 US dollar/specimen. Furthermore, the sensitivity, technical …
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