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  1. A Shiraishi1,
  2. Y Ohashi2
  1. 1Department of Ophthalmology and Regenerative Medicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, Japan
  2. 2Department of Ophthalmology, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, Japan
  1. Correspondence to Atsushi Shiraishi, Department of Ophthalmology and Regenerative Medicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan; shiraia{at}m.ehime-u.ac.jp

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We appreciate and the letter by Dr Shah et al regarding our article. They highlighted the usefulness of calcoflour white staining (CFW) and recommended that comparative trials between Fungiflora Y staining (FFY) and CFW be performed. As we have reported, we acknowledge the usefulness of CFW for detecting acanthamoeba cysts and agree on the need of a comparative study. The reason why we use FFY for detecting acanthamoeba cysts is that this dye is readily available in Japan, and the cost is about 1 US dollar/specimen. Furthermore, the sensitivity, technical …

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