Aim To demonstrate the applicability of ultrahigh-speed, ultrahigh-resolution spectral optical coherence tomography (SOCT) to cross-sectional imaging of the capsular bag in vivo.
Methods The ultrahigh-speed and ultrahigh-resolution SOCT prototype was designed and constructed at Nicolaus Copernicus University (Torun, Poland). To obtain an ultrahigh speed up to 100 000 lines/s a new spectrometer with fast CMOS line-scan camera was built. A femtosecond laser with a central wavelength of 780 nm and Δλ=160 nm enabled imaging with an axial resolution of 2.3 μm and lateral resolution of 10 μm in tissue. Lens capsules of two healthy eyes were examined with the aid of the instrument using two- and three-dimensional scanning protocols.
Results The prototype provided ultrahigh-resolution tomograms composed of 8000 A-scans with an acquisition time of 0.16 s. The quality was sufficient to evaluate the capsular bag and to estimate its thickness. It was possible to visualise a separate layer of lens epithelium, to the authors' knowledge the first such visualisation. Three-dimensional data were used to produce lens-capsule thickness maps.
Conculsions Ultrahigh-resolution, ultrahigh-speed SOCT based on a femtosecond laser allows two- and three-dimensional evaluation of a capsular bag and lens epithelium. The instrument provides new information of scientific and clinical value.
- Spectral optical coherence tomography
- lens capsule
- capsular bag
- lens and zonules
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Funding This work was supported by a European Young Investigator Award funded by European Heads of Research Councils and the European Science Foundation, Polish Ministry of Science grants for the years 2008–2012, the Foundation for Polish Science (projects Ventures and Homing).
Competing interests AK is a part-time employee of Optopol SA.
Ethics approval Ethics approval was provided by the Ethics Committee of the Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Poland.
Patient consent Obtained.
Provenance and peer review Not commissioned; externally peer reviewed.