rss
Br J Ophthalmol 94:784-789 doi:10.1136/bjo.2009.173799
  • Laboratory science

Architecture and distribution of human corneal nerves

Table 1

Acetylcholinesterase-staining solution preparation

Solutions Volume or weight
0.1 M acetate buffer pH 6.0
 0.2 M sodium acetate (1.64 g in 100 ml) 192 ml
 Distilled water 200 ml
 0.2 M acetic acid* 8 ml
Incubating solution†
 1 Acetyl thiocholine iodide 10 mg
 2 0.1 M acetate buffer (pH 6.0) 6.5 ml
 3 0.1 M sodium citrate 0.5 ml
 4 30 mM copper sulphate 1.0 ml
 5 Distilled water 1.0 ml
 6 5 mM potassium ferricyanide 1.0 ml

  • ↵* 0.2 M acetic acid was added until the correct pH was reached (pH 6.0).

  • ↵† The components were added in the order enumerated and mixed well at each stage.

This Article

  1. Abstract
  2. Full text
  3. PDF

Register for free content


Free sample
This recent issue is free to all users to allow everyone the opportunity to see the full scope and typical content of BJO.
View free sample issue >>

Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.