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Endothelin-1 production upon polyI:C stimulation of human conjunctival epithelium
  1. Mayumi Ueta1,2,
  2. Norihiko Yokoi1,
  3. Shigeru Kinoshita1
  1. 1Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  2. 2Research Center for Inflammation and Regenerative Medicine, Faculty of Life and Medical Sciences, Doshisha University, Kyoto, Japan
  1. Correspondence to Dr Mayumi Ueta, Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajiicho, Hirokoji, Kawaramachi, Kamigyoku, Kyoto 602-0841, Japan; mueta{at}koto.kpu-m.ac.jp

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We reported elsewhere that human conjunctival epithelial cells (HCjECs) express functioning interleukin (IL) 4Rα, an important molecule involved in allergic reactions, and that stimulation of these cells with IL-4 can induce the expression of various genes, including endothelin receptor type A (EDNRA),1 one of the receptors of endothelin-1 (EDN1). EDN1 is produced by airway epithelial cells.2

We also documented that polyI:C stimulation of primary HCjECs induces the secretion of inflammatory cytokines such as IL-6 and IL-8, type I interferons (IFNs) such as IFNβ, IFN-inducible proteins such as CXCL10 and CXCL11, and allergy-related proteins such as CCL5 and TSLP.3 Here we examined whether polyI:C stimulation of primary HCjECs induces the production and mRNA expression of EDN1.

This study was approved by the institutional review board of the Kyoto Prefectural University of Medicine (Kyoto, Japan). All experimental procedures were conducted in accordance with the principles set forth in the Helsinki Declaration. Primary HCjECs were harvested from conjunctival tissue obtained upon conjunctivochalasis surgery and …

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