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Original article
Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops
  1. Naoki Okumura1,
  2. Noriko Koizumi2,
  3. Morio Ueno1,
  4. Yuji Sakamoto2,
  5. Hiroaki Takahashi2,
  6. Kana Hirata2,
  7. Ryuzo Torii3,
  8. Junji Hamuro1,
  9. Shigeru Kinoshita1
  1. 1Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  2. 2Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan
  3. 3Research Center for Animal Life Science, Shiga University of Medical Science, Otsu, Japan
  1. Correspondence to Professor Noriko Koizumi, Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, 1-3, Miyakodani, Tatara, Kyotanabe 610-0321, Japan; nkoizumi{at}mail.doshisha.ac.jp

Abstract

Aim To demonstrate the efficacy of Rho-associated kinase (ROCK) inhibitor Y-27632 for corneal endothelial wound healing both in in vitro and in vivo models.

Methods As an in vitro model, cultivated cynomolgus monkey corneal endothelial cells were scraped to create a linear defect. The wound distance was then determined during a 24-h culture in the presence or absence of 10 μM of Y-27632. As an in vivo model, central corneal endothelium of Japanese white rabbits was damaged by transcorneal freezing, then 10 mM of Y-27632 was applied topically six times daily for 48 h. The wound area of the corneal endothelium was evaluated after 48 h.

Results The mean wound distance in the cultured corneal endothelial cells was significantly shorter in the Y-27632 group than in the control group. In the rabbit model, the mean wound area of the Y-27632 group was significantly smaller than that of the control group.

Conclusion This study demonstrated that ROCK inhibitor Y-27632 promotes corneal endothelial wound healing both in in vitro and in vivo.

  • Corneal endothelial cells
  • Rho kinase
  • ROCK inhibitor
  • wound healing
  • cornea
  • pharmacology
  • treatment medical
  • experimental and animal models

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Footnotes

  • Funding Supported in part by the Adaptable and Seamless Technology Transfer Program through Target-driven R&D (grant number: AS2111180G) from the Japan Science and Technology Agency.

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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