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Original article
Correlation between high-resolution optical coherence tomography (OCT) images and histopathology in an iodoacetic acid-induced model of retinal degeneration in rabbits
  1. Yasuyuki Yamauchi1,2,
  2. Tsuyoshi Agawa1,
  3. Rintaro Tsukahara1,
  4. Keisuke Kimura1,
  5. Naoyuki Yamakawa1,
  6. Masahiro Miura1,
  7. Hiroshi Goto1
  1. 1Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan
  2. 2Department of Ophthalmology, Kohsei Chuo General Hospital, Tokyo, Japan
  1. Correspondence to Dr Yasuyuki Yamauchi, Department of Ophthalmology, Tokyo Medical University Hospital, 6-7-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan; phthisis{at}nifty.com

Abstract

Background Recent research on macular disease has prompted investigation into the condition of the intersection of the inner and outer segments (IS/OS) and its relationship with retinal photoreceptor abnormalities. Because the relationship between optical coherence tomography (OCT) images and histopathology is unclear, we compared these in an iodoacetic acid (IAA)-induced model of photoreceptor degeneration in rabbits.

Methods IAA (20 mg/kg), which is toxic to photoreceptors, was injected into six coloured rabbits. After IAA administration, nine retinas were used for histopathological study: three from rabbits surviving for 1 day and six from rabbits surviving for 4 months. Four healthy rabbit retinas served as controls. OCT images were taken before euthanasia.

Results In the controls, OCT images revealed the IS/OS as a clear, straight line. In rabbits surviving for 1 day, the structure of the photoreceptor IS/OS was destroyed and the IS/OS boundary was not visible. In rabbits surviving for 4 months, the IS was still preserved, but the structure of the OS was destroyed or partially disorganised, and the IS/OS was observed as a wavy, broken line on the OCT images.

Conclusion The IS/OS on the OCT images reflected the histopathology of the inner and outer segments in a photoreceptor degeneration model.

  • IS/OS
  • OCT
  • histopathology
  • iodoacetic acid
  • retina
  • pathology
  • imaging
  • experimental and animal models

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Footnotes

  • Competing interests None to declare.

  • Ethics approval Obtained from the Tokyo Medical University Institutional Animal Care and Use Committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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