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Original article
Correlation between high-resolution optical coherence tomography (OCT) images and histopathology in an N-methyl-N-nitrosourea–induced retinal degeneration rat model
  1. Yasuyuki Yamauchi,
  2. Keisuke Kimura,
  3. Tsuyoshi Agawa,
  4. Rintaro Tsukahara,
  5. Maki Mishima,
  6. Naoyuki Yamakawa,
  7. Hiroshi Goto
  1. Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan
  1. Correspondence to Yasuyuki Yamauchi, Department of Ophthalmology, Tokyo Medical University Hospital, 6-7-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan; phthisis{at}nifty.com

Abstract

Background Recent research on macular diseases has prompted investigations into the condition of the intersection between the inner and outer segments (IS/OS), and its relationship with retinal photoreceptor abnormalities. Because the correlation between optical coherence tomography (OCT) images and histopathology is unclear, the authors compared them in an N-methyl-N-nitrosourea (MNU)-induced photoreceptor degeneration rat model.

Methods MNU (60 mg/kg), which is toxic to photoreceptors, was injected in 12 Brown Norway rats. After MNU administration, three rats were used per histopathological study 3 h, 6 h, 24 h and 1 week after the injections. Two healthy rats served as controls. OCT images were taken before euthanisation.

Results 3 h after the MNU injections, the IS and OS were oedematous, but the IS/OS borderline was recognised histopathologically, and the IS/OS was depicted on the OCT images. Six hours after injections, the OS were preserved, but the IS structures were destroyed or partially disorganised histopathologically, and the IS/OS was not observed on the OCT images. Twenty-four hours after the injections, the IS and OS were totally disorganised histopathologically, and the IS/OS was not depicted on the OCT images.

Conclusion The IS structure might be the origin of the IS/OS on OCT images.

  • IS/OS
  • OCT
  • histopathology
  • N-methyl-N-nitrosourea
  • retina
  • pathology
  • imaging
  • experimental animal models

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Footnotes

  • Linked article 186718.

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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