Background Let-7 microRNA is an important regulator of cellular ageing and tissue senescence. The objective of this study is to evaluate the expression of let-7a/let-7b/let-7c microRNAs in human age-related cataracts.
Aim To evaluate the correlation among the severity of lens opacity, the level of let-7a/let-7b/let-7c microRNA expression and patient age in the context of age-related cataracts.
Methods The authors evaluated the mRNA level of let-7a/let-7b/let-7c microRNA in lens epithelia obtained from 174 eyes with age-related cataracts. The authors also recorded the patient age and the severity of lens opacity as classified according to the modified version of the Lens Opacities Classification System version III.
Results Let-7b microRNA expression was demonstrated to be positively associated with patient age (R=0.472; p<0.001). A positive correlation was also observed between higher N, C and P cataract scores and higher expression of let-7b microRNA in patients with age-related cataracts (p<0.001). However, no significant correlation was observed between the let-7a and let-7c microRNA expression levels and either the severity of lens opacity or the patient age.
Conclusion These findings suggest that microRNAs play a role in age-related cataracts. A local let-7b microRNA increase may represent a risk factor in the formation of age-related cataracts.
- Lens and zonules
- stem cells
- eye lids
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This paper has not been presented at any conference.
Funding This work was assisted in part by the Division of Experimental Surgery of the Department of Surgery, Taipei Veterans General Hospital. This study was supported by the National Science Council (98-2314-B-341-001-MY3, 99-2314-B-075-005- MY3), the Joint Projects of UTVGH (VGHUST VN100-03), Taipei Veterans General Hospital (C1-099/ER3-001), Taipei City Hospital (96/97/98/099XDAA00102/10001-62-042), Shin-Kong Wu Ho-Su Memorial Hospital (SKH-8302-97-DR-25, SKH-8302-98-DR-26), Zuoying Armed Forces General Hospital (9502/9696/9719), Cheng-Hsin General Hospital (CHGH-100-01, 100-19, 100-20, 100-22, 100-40) and Yen-Tjing Ling Medical Foundation (96/97/98). Taiwan.
Competing interests None.
Patient consent Obtained.
Ethics approval Ethics approval was provided by Taipei City Hospital.
Provenance and peer review Not commissioned; externally peer reviewed.