Menu

rss
Br J Ophthalmol 96:1034-1037 doi:10.1136/bjophthalmol-2012-301624
  • Laboratory science
  • Original article

The effect of gravitational force on limbal stem cell growth

  1. Harminder Singh Dua1
  1. 1Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham, UK
  2. 2Department of Ophthalmology, Aleppo University, Aleppo, Syria
  3. 3Department of Ophthalmology, Hashmanis Hospital, Karachi, Pakistan
  4. 4College of Medicine, University of Basrah, Basrah, Iraq
  5. 5Research institute of Ophthalmology, Cairo, Egypt
  1. Correspondence to Dr Harminder Singh Dua, Division of Ophthalmology and Visual Sciences, B Floor, Eye ENT Centre, Queens Medical Centre, University Hospital, Derby Road, Nottingham NG7 2UH, UK; harminder.dua{at}nottingham.ac.uk

  1. Contributors AM and KH are joint first authors.

  • Accepted 16 April 2012
  • Published Online First 19 May 2012

Abstract

Aim To evaluate the effect of gravity on corneal epithelial cell migration in vitro.

Methods Fourteen donor peripheral corneoscleral rims were used. Twenty explants were chosen of which 10 were placed vertically and 10 were placed horizontally during culture. Analyses were performed to investigate the effect of gravity on epithelial growth by measuring the extent of epithelial cell growth above and below the horizontal meridian and counting the total number of cells using a haemocytometer.

Results There was no statistically significant difference in cell growth between the explants that were placed horizontally and vertically. However, in the vertical explant group the cells grew preferentially towards the 6 o'clock direction, possibly as a result of gravity.

Conclusions Gravitational forces may influence cell migration in vitro. This could be of significance in the planning of limbal transplantation, because a superior graft may be more likely to succeed than a gravitationally challenged inferior graft.

Footnotes

  • Competing interests None.

  • Patient consent Obtained.

  • Ethics approval Ethics approval for this study was obtained from the local ethics dommittee no 07/H0403/140.

  • Provenance and peer review Not commissioned; externally peer reviewed.

This Article

  1. Abstract
  2. Full text
  3. PDF
  4. All Versions of this Article:
    1. bjophthalmol-2012-301624v1
    2. 96/7/1034 most recent

Responses

  1. Submit a response
  2. No responses published

Social bookmarking

Register for free content


Free sample
This recent issue is free to all users to allow everyone the opportunity to see the full scope and typical content of BJO.
View free sample issue >>

Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.