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Expression of prostaglandin F receptor in scleral and subconjunctival tissue
  1. Kojiro Imai1,
  2. Mayumi Ueta1,2,
  3. Kazuhiko Mori1,
  4. Morio Ueno1,
  5. Yoko Ikeda1,
  6. Toru Oga3,
  7. Norihiko Yokoi1,
  8. Katsuhiko Shinomiya1,
  9. Shuh Narumiya3,
  10. Shigeru Kinoshita1
  1. 1Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  2. 2Research Center for Inflammation and Regenerative Medicine, Faculty of Life and Medical Sciences, Doshisha University, Kyoto, Japan
  3. 3Department of Pharmacology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  1. Correspondence to Dr Mayumi Ueta, Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokoji-agaru, Kawaramachi-dori, Kamigyo-ku, Kyoto 602-0841, Japan; mueta{at}koto.kpu-m.ac.jp

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Prostaglandin F2α (PGF) analogues eye drops are regarded as a first choice for the treatment of glaucoma. The hypotensive action of PGF analogues is thought to be attributed to an increase in uveoscleral outflow.1 However, the underlying mechanisms have yet to be well defined. The purpose of this present study was to examine prostaglandin F receptor (FP) localisation in ocular tissue.

All experiments were conducted in accordance with the principles set forth in the Declaration of Helsinki. The expression of FP was examined by X-gal staining in ocular tissues of FP knockout mice carrying the β-galactosidase gene at the FP loci (Ptgfr−/− mice)2 and reverse transcription PCR of human conjuncitival and scleral fibroblasts (see supplementary methods, published online only).

First, FP localisation was examined using Ptgfr−/− mice in which the β-galactosidase gene was ‘knocked-in’ at the FP gene. …

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