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- diagnostic tests/investigation
- experimental and laboratory
- human conjunctival fibroblast
- human scleral fibroblast
- ocular surface
- prostaglandin F receptor (FP)
- treatment surgery
Prostaglandin F2α (PGF2α) analogues eye drops are regarded as a first choice for the treatment of glaucoma. The hypotensive action of PGF2α analogues is thought to be attributed to an increase in uveoscleral outflow.1 However, the underlying mechanisms have yet to be well defined. The purpose of this present study was to examine prostaglandin F receptor (FP) localisation in ocular tissue.
All experiments were conducted in accordance with the principles set forth in the Declaration of Helsinki. The expression of FP was examined by X-gal staining in ocular tissues of FP knockout mice carrying the β-galactosidase gene at the FP loci (Ptgfr−/− mice)2 and reverse transcription PCR of human conjuncitival and scleral fibroblasts (see supplementary methods, published online only).
First, FP localisation was examined using Ptgfr−/− mice in which the β-galactosidase gene was ‘knocked-in’ at the FP gene. …