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  1. Andrew C Browning1,
  2. Elizabeth Anne Stewart2,
  3. Winfried M Amoaku2
  1. 1Department of Ophthalmology, Royal Victoria Infirmary, Newcastle-upon-Tyne, UK
  2. 2Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham, UK
  1. Correspondence to Winfried M Amoaku, Division of Ophthalmology and Visual Sciences, University of Nottingham, B Floor, Eye and ENT Building, Queen's Medical Centre, Nottingham NG7 2UH, UK; winfried.amoaku{at}nottingham.ac.uk

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We thank Leach et al for their interest in our recent publication ‘Comparative gene expression profiling of human umbilical vein endothelial cells (HUVEC) and ocular vascular endothelial cells.’

Our experiments are based on monolayers of endothelial cells (ECs) grown under similar conditions for comparison. The results show significant differences between retinal and choroidal microvascular ECs and HUVEC. This is not surprising as particular diseases have particular predilection for specific vascular beds even within the same organ. The investigation of proliferation and IGF signalling in the microvascular ECs was confirmatory to …

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