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Sensitivity and specificity of the AdenoPlus point-of-care system in detecting adenovirus in conjunctivitis patients at an ophthalmic emergency department: a diagnostic accuracy study
  1. K Y Ronald Kam1,2,
  2. Hon Shing Ong1,
  3. Catey Bunce3,
  4. Lola Ogunbowale1,
  5. Seema Verma1
  1. 1Moorfields Eye Hospital, London, UK
  2. 2Faculty of Medicine, Department of Surgery and Cancer, Imperial College London, London, UK
  3. 3National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, UK
  1. Correspondence to Seema Verma, Moorfields Eye Hospital, 162 City Road, London EC1V 2PD, UK; seema.verma{at}moorfields.nhs.uk

Abstract

Background To estimate the diagnostic accuracy (sensitivity and specificity) of the AdenoPlus point-of-care adenoviral test compared to PCR in an ophthalmic accident and emergency service. These findings were compared with those of a previous study.

Methods This was a prospective diagnostic accuracy study on 121 patients presenting to an emergency eye unit with a clinical picture of acute adenoviral conjunctivitis. AdenoPlus testing was carried out on one eye of each patient and a PCR analysis was also performed on a swab taken from the same eye. AdenoPlus and PCR results were interpreted by masked personnel. Sensitivity and specificity for the AdenoPlus test were calculated using PCR results as the reference standard.

Results 121 patients were enrolled and 109 met the inclusion criteria. 43 patients (39.4%) tested positive for adenovirus by PCR analysis. The sensitivity of the AdenoPlus swab in detecting adenovirus was 39.5% (17/43, 95% CI 26% to 54%) and specificity was 95.5% (63/66, 95% CI 87% to 98%) compared to PCR.

Conclusions The AdenoPlus test has a high specificity for diagnosing adenoviral conjunctivitis, but in this clinical setting, we could not reproduce the high sensitivity that has been previously published.

  • Conjunctiva
  • Diagnostic tests/Investigation
  • Infection

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