Aims: To determine if neural elements are present in sub-and epiretinal PVR membranes as well as in diabetic, fibrovascular membranes removed from patients during vitrectomy surgery.
Methods: Human sub- and epiretinal membranes of varying durations were immunolabeled with different combinations of antibodies to GFAP, vimentin, neurofilament protein and laminin.
Results: Anti-neurofilament labeled neurites from presumptive ganglion cells were frequently found in epiretinal membranes and occasionally found in subretinal membranes. In addition the neurites were only observed in regions that also contained glial processes.
Conclusions: These data demonstrate that neuronal processes are commonly found in human peri-retinal cellular membranes similar to what has been demonstrated in animal models. These data also suggest that glial cells growing out of the neural retina form a permissive substrate for neurite growth and thus may hold clues to factors that support this growth.
- Muller cells
- epiretinal membrane
- ganglion cells
- retinal detachment
- subretinal membrane