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Positive association of pigment epithelium-derived factor (PEDF) with total anti-oxidant capacity in the vitreous fluid of patients with proliferative diabetic retinopathy
  1. Masahiko Yokoi (zoyokoi.tdr{at}keijinkai.or.jp),
  2. Sho-ichi Yamagishi (shoichi{at}med.kurume-u.ac.jp),
  3. Akari Saito (akari{at}med.hokudai.ac.jp),
  4. Yumiko Yoshida (yoshida_yumiko{at}kurume-u.ac.jp),
  5. Takanori Matsui (matsui_takanori{at}med.kurume-u.ac.jp),
  6. Wataru Saito,
  7. Shigeki Hirose (s-hirose{at}med.hokudai.ac.jp),
  8. Kazuhiro Ohgami (kohgami{at}med.hokudai.ac.jp),
  9. Manabu Kase (kase{at}keijinkai.or.jp),
  10. Shigeaki Ohno (sohno{at}med.hokudai.ac.jp)
  1. Department of Ophthalmology, Teine Keijinkai-Hospital, Japan
  2. Department of Medicine, Division of Cardiovascular Medicine, Kurume University School of Medicine, Japan
  3. Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Japan
  4. Department of Medicine, Division of Cardiovascular Medicine, Kurume University School of Medicine, Japan
  5. Department of Medicine, Division of Cardiovascular Medicine, Kurume University School of Medicine, Japan
  6. Hokkaido University Graduate School, Japan
  7. Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Japan
  8. Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Japan
  9. Department of Ophthalmology, Teine-Keijinkai Hospital, Japan
  10. Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Japan

    Abstract

    Background: We have recently found that pigment epithelium-derived factor (PEDF), a glycoprotein with potent neuronal differentiating activity, inhibits advanced glycation end product-induced retinal hyperpermeability and angiogenesis through its anti- oxidative properties, suggesting that PEDF may exert beneficial effects on diabetic retinopathy by acting as an endogenous anti-oxidant. However, the interrelationship between PEDF and total anti-oxidant capacity in the eye remains to be elucidated. In this study, we determined vitreous PEDF and total anti- oxidant levels in patients with proliferative diabetic retinopathy (PDR), and investigated the relationship between them.

    Methods: Vitreous levels of PEDF and total anti- oxidant capacity were measured by an ELISA in 39 eyes of 36 diabetic patients with PDR and in 29 eyes of 29 non- diabetic control subjects.

    Results: Vitreous levels of total anti-oxidant capacity were significantly lower in diabetic patients with PDR than in control subjects (0.16±0.05 mmol/l vs 0.24±0.09 mmol/l, p<0.001). PEDF levels were positively correlated with total anti-oxidant status in the vitreous of patients with PDR (r=0.37, p<0.05) and control subjects (r=0.41, p<0.05). Further, vitreous levels of PEDF in PDR patients without vitreous hemorrhages (VH(-)) were significantly (p<0.05) decreased, compared with those in the control or PDR patients with vitreous hemorrhage (VH(+)) (PDR VH(-), 4.5±1.1 μg/ml; control, 7.4±4.1 μg/ml; PDR VH(+) 8.5±3.6 μg/ml)

    Conclusion: This study demonstrates that PEDF levels are associated with total anti-oxidant capacity of vitreous in humans. Our observations suggest that PEDF may act as an endogenous anti-oxidant in the eye and could play a protective role against PDR.

    • oxidative stress
    • pigment epithelium-derived factor
    • proliferative diabetic retinopathy
    • total anti-oxidant capacity

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