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Vitreoretinal surgery using Bromphenol blue as a vital stain: Evaluation of staining characteristics in humans
  1. Christos Haritoglou (christos.haritoglou{at}med.uni-muenchen.de),
  2. Ricarda Schumann,
  3. Rupert Strauss,
  4. Siegfried Priglinger,
  5. Aljoscha S Neubauer,
  6. Anselm Kampik
  1. Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany
  2. Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany
  3. Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany
  4. Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany
  5. Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany
  6. Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstr. 8, 80336 Munich, Germany

    Abstract

    Purpose: To evaluate the staining characteristics of bromphenolblue used during vitreoretinal surgery in humans.

    Patients and methods: 13 patients with epiretinal membranes were included. Before and after surgery a complete clinical examination including best corrected visual acuity, funduscopy, fluorescein angiography, OCT (Stratus), Goldmann perimetry and multifocal ERG as well as photography of the macular area was performed. Bromphenolblue was used in concentrations of 0.2% in most patients. Removed epiretinal tissue was evaluated using electron microscopy.

    Results: Using dye concentrations of 0.2% a good demarcation of epiretinal membranes was seen in 11/13 patients. Staining of vitreous remnants at the vitreous base was seen in all patients. No dye related adverse events were seen during follow up in the functional tests (VA, ERG, perimetry) performed. Histological evaluation of epiretinal membranes showed unremarkable aspects of epiretinal cellular layers and unremarkable retinal surface of the ILM.

    Conclusion: Bromphenolblue appears to be a very helpful and safe tool in posterior segment surgery. The staining characteristics need to be further evaluated in prospective study settings and larger numbers of patients.

    • bromphenol blue
    • vital dyes
    • vitreoretinal surgery

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