Background/aims: The repeatability of an automatic system for estimation of endothelial cell density (ECD) from microscopy images in donor corneas was assessed.
Methods: A computer program for the automatic ECD estimation from frequency analysis was previously presented. An evaluation of its repeatability was performed on a data set containing 200 corneas, by assessing accuracy and precision of automatic vs. manual values. For each cornea, between 2 and 21 images (1536 total) at 100x for automatic ECD and one image at 200x for manual ECD were available.
Results: Accuracy of automatic ECDs was −45 ± 99 cells/mm2 (−2% ± 4%). Precision of repeated automatic ECDs on the same cornea was 62 ± 30 cells/mm2 (3% ± 1%). The algorithm was also capable of identifying all non-processable images.
Conclusion: The proposed automatic technique proved to be reliable for its routine use in a typical eye-bank setting like the one considered here.
- endothelial cell density
- eye bank
- image analysis