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Pars planitis is associated with an increased frequency of effector-memory CD57+ T cells
  1. Miguel Pedroza-Seres,
  2. Marisela Linares,
  3. Stephanie Voorduin,
  4. Enrique Rojas-Ramos,
  5. Ricardo Lascurain,
  6. Yonathan Garfias,
  7. Maria C Jimenez-Martinez (jmaricarmen{at}yahoo.com)
  1. Research Unit and Uvea Department, Institute of Ophthalmology Fundación Conde de Valenciana, Mexico
  2. Research Unit and Uvea Department, Institute of Ophthalmology Fundación Conde de Valenciana, Mexico
  3. Research Unit and Uvea Department, Institute of Ophthalmology Fundación Conde de Valenciana, Mexico
  4. Departamento de Inmunología Clínica y Alergia, Hospital 1o. de Octubre, ISSSTE, Mexico
  5. Departamento Bioquímica, Fac. Med. UNAM., Mexico
  6. Research Unit and Uvea Department, Institute of Ophthalmology Fundación Conde de Valenciana, Mexico
  7. Research Unit and Uvea Department, Institute of Ophthalmology Fundación Conde de Valenciana, Mexico

    Abstract

    Aim: To evaluate the frequency, phenotype and the potential function of CD57+ T cell subsets in patients with pars planitis(PP).

    Methods: CD4+CD57+ and CD8+CD57+ T cells were quantitated in peripheral blood from 15 patients with PP and 15 healthy controls. To evaluate the phenotype and potential function of CD57+ T cell subsets CCR7, CD27, CD28, CD45RA, CD45RO, intracellular IFN-gamma, IL-4, perforin and granzyme-A expression were assessed by flow cytometry.

    Results: CD57+ T cells subsets were increased in patients with PP(p=0.002). The majority of CD4+CD57+ T cells were CCR7-CD27-CD28-CD45RO+, while the most CD8+CD57+ T cells were CCR7-CD27-CD28-CD45RA+. The number of cells positive for intracellular IFN-g and IL-4 was higher in the CD57+ T cell populations. A greater number of CD8+CD57+ T cells than CD8+CD57- T cells were positive to perforin(p=0.006) and granzyme-A(p=0.01).

    Conclusions: CD57+ T cells had a phenotype associated with peripheral memory (CCR7-CD27-CD28-). Cytokine production by CD57+ T cells suggests that these cells may play a role in helper cell regulation. High expression of intracellular proteins involved in cytotoxicity suggests that CD8+CD57+ T cells may play an effector role. Taken together, this study proposes that CD57+ T cells function as memory-effector T cell subsets during PP pathogenesis.

    • CD57 T cells
    • Pars planitis
    • effector-memory cells
    • intermediate uveitis

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