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The genotypic characterization of Acanthamoeba isolates from human ocular samples
  1. Helene Yera (helene.yera{at}cch.aphp.fr),
  2. Oana Zamfir,
  3. Tristan Bourcier,
  4. Eric Viscogliosi,
  5. Christophe Noël,
  6. Jean Dupouy-Camet,
  7. Christine Chaumeil
  1. Laboratoire de Parasitologie Mycologie, Hôpital Cochin, AP-HP, Université Paris Descart, France
  2. Laboratoire, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France
  3. Service 5, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France
  4. Inserm, U547, Institut Pasteur, Lille, France
  5. Institute for Research on Environment and Sustainability, University of Newcastle upon Tyne, France
  6. Laboratoire de Parasitologie Mycologie, Hôpital Cochin, AP-HP, Université Paris Descart, France
  7. Laboratoire, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France

    Abstract

    Background: We characterized 37 amoebae cultured from corneal scrapings, contact lenses or lens case solutions of patients with suspected Acanthamoeba keratitis.

    Methods: The isolates were identified by their morphology and by PCR targeting the Acanthamoeba nuclear small-subunit (SSU) rRNA gene. Acanthamoeba isolates were genotyped by DNA sequence analysis.

    Results: The 37 isolates comprised 35 Acanthamoeba, one Hartmannella and one Vahlkampfia. Ten Acanthamoeba isolates were shown to be responsible for keratitis.

    Conclusion: Genotype T4 was the only Acanthamoeba genotype responsible for keratitis in this study, and represented 79% of nonpathogenic isolates.

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