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Prevalence of Herpes Simplex Virus Type 1 Glycoprotein G (gG) and gI Genotypes in Patients with Herpetic Keratitis
  1. Rui Duan (r.duan{at}erasmusmc.nl),
  2. Jessica M van Dun (j.vandun{at}erasmusmc.nl),
  3. Lies Remeijer (l.remeijer{at}planet.nl),
  4. Martin Siemerink (m.siemerink{at}oogziekenhuis.nl),
  5. Paul G Mulder (p.mulder{at}erasmusmc.nl),
  6. Peter Norberg (peter.norberg{at}microbio.gu.se),
  7. Albert D Osterhaus (a.osterhaus{at}erasmusmc.nl),
  8. Georges M Verjans (g.verjans{at}erasmusmc.nl)
  1. Erasmus Medical Center, Netherlands
  2. Erasmus Medical Center, Netherlands
  3. Rotterdam Eye Hospital, Netherlands
  4. Rotterdam Eye Hospital, Netherlands
  5. Erasmus Medical Center, Netherlands
  6. University of Gotenborg, Sweden
  7. Erasmus Medical Center, Netherlands
  8. Erasmus Medical Center, Netherlands

    Abstract

    Aim: Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG), and US7 (encoding gI) of clinical herpes simplex virus type 1 (HSV-1) isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).

    Methods: Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes gG and gI encoding genes US4 and US7, respectively.

    Results: Genotype B was more frequently expressed by the corneal HSV-1 isolates compared to genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.

    Conclusions: The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.

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