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Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents
  1. Noemi Lois (noemilois{at}aol.com),
  2. Rosie Dawson (r.dawson{at}abdn.ac.uk),
  3. John Townend (j.townend{at}abdn.ac.uk),
  4. Alastair D McKinnon (a.d.mckinnon{at}abdn.ac.uk),
  5. Gillian C Smith,
  6. Rob van't Hof,
  7. Nico Van Rooijen (nvanrooijen{at}clodronateliposomes.org),
  8. John V Forrester (j.forrester{at}abdn.ac.uk)
  1. Ophthalmology Department, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom
  2. Ophthalmology Department, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom
  3. Department of Public Health, University of Aberdeen, Scotland, United Kingdom
  4. Ophthalmology Department, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom
  5. Ophthalmology Department, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom
  6. Bone Research Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom
  7. Department of Cell Biology and Immunology, Faculty of Medicine, Free University, Amsterdam, Netherlands
  8. Ophthalmology Department, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, United Kingdom

    Abstract

    Purpose: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO).

    Methods: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl2MDP-lip-treated group, n=10) or phosphate-buffered saline (control group, n=8) one day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. Fisher’s exact test and randomization test were used to assess statistically differences between groups.

    Results: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl2MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring's ring formation showed no statistically significance between groups (p= 0.27, p= 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the center of the posterior capsule was found in the Cl2MDP-lip-treated group (p= 0.009).

    Conclusion: Depletion of macrophages was accompanied by a reduction in LEC in the center of the posterior capsule in rodents.

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