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Inhibitory Effect of Rapamycin and Dexamethasone on Production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada Patients
  1. Ke Yang (yangke_hot{at}yahoo.com.cn),
  2. Jianguo Wen (jgwen{at}zzu.edu.cn),
  3. Xiaoli Liu (lili4451{at}sina.com),
  4. Aize Kijlstra (aize.kijlstra{at}wur.nl),
  5. Lina Chen (docchenln{at}yahoo.com),
  6. Wei Chi (cwcj2002{at}163.com),
  7. Hongyan Zhou (hyz010203{at}126.com),
  8. Xianghun Huang (hxkun{at}163.com),
  9. Peizeng Yang (peizengy{at}126.com)
  1. First Clinical Hospital of ZhengZhou University and State Key Laboratory of Ophthalmology, China
  2. The First Clinical Hospital of ZhengZhou University, China
  3. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, China
  4. Eye Research Institute Maastricht, Department of Ophthalmology, University Hospital Maastricht, Netherlands
  5. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, China
  6. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, China
  7. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, China
  8. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, China
  9. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, China

    Abstract

    Aims: To evaluate the effect of rapamycin (RAPA) and Dexamethasone (DEX) on the production of IL-17 and IFN-gamma by peripheral blood mononuclear cells (PBMCs) from Vogt-Koyanagi-Harada (VKH) patients and healthy individuals.

    Methods: Blood samples were drawn from 10 active VKH patients and 10 healthy individuals. PBMCs were cultured with or without anti-CD3 and anti-CD28 antibodies in the presence or absence of different concentrations of RAPA or DEX for 72 hours. IL-17 and IFN-gamma concentrations in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA).

    Results: The expression of IL-17 and IFN-gamma was significantly increased in active VKH patients compared with that in normal controls. Both RAPA and DEX could significantly inhibit the production of IL-17 and IFN-gamma by PBMCs from patients and normal controls. RAPA could completely inhibit IL-17 production at a dosage of 10 ng/ml, but only partially suppressed IFN-gamma production even at a much higher concentration (1000 ng/ml). DEX inhibited the production of both IL-17 and IFN-gamma approximately by 70%.

    Conclusions: This study indicates that both RAPA and DEX inhibit the production of IL-17 and IFN-gamma by PBMCs. RAPA is much stronger in inhibiting the production of IL-17 than DEX.

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