Aims: Evaluate the histopathology of neovascular tufts and vitreous samples collected from diabetic patients.
Methods: Vitreous samples and neovascular tufts were collected from type 1 (n=13) and type 2 diabetics (n=17) with proliferative retinopathy and controls with macular hole (n=5). Neovessels were analyzed using immunohistochemistry and vitreous samples with Enzyme-linked immunosorbent assay (ELISA). Main outcome measure was to find differences in growth factors in proliferative retinopathy between type 1 and type 2 diabetics.
Results: VEGF-A was most strongly present in the samples in type 1 diabetics. In type 2 diabetics, VEGF-D was more abundantly present than in type 1 diabetics. Also ANG-2 was abundantly present. Macrophages and nuclear factor kappa B (NFκB) were found indicating the presence of an inflammatory process in the neovascular tissues.
Conclusions: VEGF-A and ANG-2 are equally important in neovascular process in both type 1 and type 2 diabetics. In type 2 diabetics, VEGF-D is abundantly present. To achieve better control of diabetic retinopathy, it might be beneficial to target also against the actions of ANG-2 and VEGF-D.