Article Text

other Versions

PDF
Cultivation of Lacrimal Gland Acinar Cells in a Microgravity Environment
  1. Stefan Schrader (mail{at}stefanschrader.de),
  2. Claudia Kremling (ckremling{at}uni-luebeck.de),
  3. Matthias Klinger (klinger{at}anat.uni-luebeck.de),
  4. Horst Laqua (hlaqua{at}uni-luebeck.de),
  5. Gerd Geerling (g.geerling{at}augenklinik.uni-wuerzburg.de)
  1. Department of Ophthalmology, University of Luebeck, Germany
  2. Department of Ophthalmology, University of Luebeck, Germany
  3. Department of Anatomy, University of Luebeck, Germany
  4. Department of Ophthalmology, University of Luebeck, Germany
  5. Department of Ophthalmology, Julius-Maximilian-University Wuerzburg, Germany

    Abstract

    Background: A Rotary Cell Culture System (RCCS) allows the creation of a microgravity environment of low shear force, high-mass transfer, and 3-dimensional cell culture of various cell types. Aim of this study was to evaluate the growth pattern and the secretory function of rabbit lacrimal gland acinar cells in a microgravity environment using a RCCS.

    Methods: Lacrimal gland acinar cells from male New Zealand White rabbits were isolated and cultured in a RCCS up to 28 days. Cells were analysed by light and electron microscopy and apoptosis was assessed by the TUNEL-Assay at day 7, 14, 21 and 28. Secretory function was tested by measuring the ß-hexosaminidase activity.

    Results: After 7 days of culture, spheroidal aggregates were found inside the RCCS. The spheroids consisted of acinus-like cell conglomerates. Apoptotic centers inside the spheroids were observed at all time points by means of the TUNEL-Assay. Evaluation of the secretory function revealed ß-hexosaminidase release after carbachol stimulation which decreased over the culture period.

    Conclusion: A simulated microgravity environment promotes the development of three dimensional cell spheroids containing viable acinar cells up to 28 days. Due to the evolving central apoptosis, it is unlikely that such simple three dimensional cell communities can serve as tissue equivalents for clinical transplantation, but they promise opportunities for further applications in basic and applied cell research on lacrimal gland cells.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.