Possible Contribution of Hyalocytes to Idiopathic Epiretinal Membrane Formation and Its Contraction
- Ri-ichiro Kohno (kreach{at}med.kyushu-u.ac.jp),
- Yasuaki Hata (hatachan{at}med.kyushu-u.ac.jp),
- Shuhei Kawahara (kawashuu{at}med.kyushu-u.ac.jp),
- Takeshi Kita (kita{at}eye.med.kyushu-u.ac.jp),
- Ryoichi Arita (r-arita{at}med.kyushu-u.ac.jp),
- Yasutaka Mochizuki (mochi{at}med.kyushu-u.ac.jp),
- Lloyd Paul Aiello (lloydpaul.aiello{at}joslin.harvard.edu),
- Tatsuro Ishibashi (ishi{at}med.kyushu-u.ac.jp)
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Ophthalmology, Harvard Medical School, United States
- Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Japan
- Published Online First 7 May 2009
Abstract
Purpose: To address the cellular components and the contractile mechanisms of idiopathic epiretinal membrane (ERM).
Methods: Ten surgically removed ERMs were fixed in 4% paraformaldehyde and analyzed by whole-mount immunohistochemistry with anti-GFAP and αSMA antibodies. Type I collagen gel contraction assay, an established wound healing assay in vitro, was performed using cultured bovine hyalocytes or normal human astrocytes (NHA) to evaluate the contractile property of the cells in the presence of TGF-β2. The expression of αSMA was also analyzed by Western blot analysis to examine myofibroblastic transdifferentiation of the cells. Vitreous-induced collagen gel contraction was also evaluated.
Results: All membranes were composed of αSMA immunopositive cells in contracted foci and GFAP immunopositive cells in the periphery. No apparent double positive cells were observed in any membranes examined. Cultured hyalocytes showed overexpression of αSMA and hypercontraction of collagen gels in response to TGF-β2, while glial cells showed marginal change. The vitreous from ERM patients also caused overexpression of αSMA and hypercontraction of the gels embedding hyalocytes, which were almost completely inhibited in the presence of anti-TGF-β2 neutralizing antibody.
Conclusions: Hyalocytes might be one of the critical components of ERM mediating its contractile property through the effect of TGF-β2 in the vitreous fluid.
