Article Text

other Versions

Use of ultrasonic pachymetry for measurement of changes in corneal thickness in mouse corneal transplant rejection
  1. Thomas H Flynn1,*,
  2. Masaharu Ohbayashi2,
  3. Maria Dawson1,
  4. Mohammad Siddique1,
  5. Santa J Ono2,
  6. Daniel F Larkin3
  1. 1 Institute of Ophthalmology, United Kingdom;
  2. 2 Emory University, United States;
  3. 3 Moorfields Eye Hospital, United Kingdom
  1. Correspondence to: Tom Flynn, /, /, /, /, United Kingdom; t.flynn{at}


Background/aims: Diagnosis of rejection in the mouse model of corneal transplantation is based on subjective judgement of loss of graft transparency. The aims of this study were to (i) evaluate a pachymetry technique to measure changes in mouse corneal thickness, and (ii) to correlate increases in transplant thickness with clinical and histological correlates of rejection.

Mehtods: Orthotopic corneal allografts (C57BL/6 strain donor) and syngeneic grafts were performed in A/J mice. Graft transparency was graded and corneal thickness measured by pachymetry on alternate days. Transverse sections of donor cornea excised from eyes representative of clinical opacity grades 1- 4 were prepared, photographed, graft section thickness measured and stromal graft-infiltrating cells counted. Intraobserver and interobserver variation in pachymetry was statistically tested.

Results: Graft thickness, as measured by pachymetry, increased with each clinical opacity grade. Thickness for opacity grades 0, 1 and 2 was in all recipients less than 300 μm. Graft thickness for grades 3 and 4 was in all cases greater than 300 μm. For measurements up to 400 μm there was a good correlation between thickness as measured by in vivo pachymetry and in histopathological sections. Mean interobserver bias was -11.35 μm while mean intraobserver bias was +3.96 μm. Stromal cellularity increased with increasing corneal thickness up to approximately 300 μm.

Conclusion: In vivo graft pachymetry provides a new and reliable way to objectively diagnose rejection in the mouse model of corneal transplantation.

Statistics from


    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.