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Architecture and distribution of human corneal nerves.
  1. Mouhamed Al-Aqaba1,
  2. Usama Fares1,
  3. Hanif Suleman1,
  4. James Lowe1,
  5. Harminder Singh Dua2,*
  1. 1 University of Nottingham, United Kingdom;
  2. 2 University Hospital, United Kingdom
  1. Correspondence to: Harminder Singh Dua, Ophthalmology, University Hospital, Division of Ophthalmology and Visual Sciences, B Floor, Eye ENT Centre, University Hospital, Queens Medical Centre, Derby Road, Nottingham, NG7 2UH, United Kingdom; harminder.dua{at}


Aims: To comprehensively study the gross anatomy of human corneal innervation.

Methods: Twenty one specimen including 12 normal human corneas from 7 deceased patients, two eye bank corneo-scleral buttons, two eye bank corneo-scleral rims and 5 post-surgical specimens from 3 patients with keratoconus were studied. Corneal whole mounts were stained for cholinesterase enzyme using the Karnovsky & Roots direct coloring thiocholine modification of Acetylcholinestrase (AchE) technique.

Results: Approximately 44 thick nerve bundles were found to enter the human cornea in a relatively equal distribution round the limbus and move randomly toward the central cornea. At the mid-peripheral zone, anterior stromal nerves showed a characteristic budding and branching pattern. After passing through Bowman's zone they were noted to terminate into bulb like thickenings from which multiple sub-basal nerves arose. The perforation sites were predominantly located in the mid-peripheral cornea. The orientation of sub-basal nerves was mainly vertical at their origin from the perforation sites. Nerves from all directions converged toward the infero-central cornea to form a characteristic clockwise whorl pattern.

Conclusions: This study provides a comprehensive account of the architecture and distribution of nerves in the human cornea. It reconciles some of the existing information obtained from other modalities of investigation and identifies some novel features which provide a more complete picture of corneal innervation.

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