Article Text

other Versions

PDF
Letter
Reverse big bubble: a new technique for preparing donor tissue of Descemet membrane endothelial keratoplasty
  1. S Zarei-Ghanavati,
  2. H Khakshoor,
  3. M Zarei-Ghanavati
  1. Mashhad University of Medical Sciences, Mashhad, Iran
  1. Correspondence to Dr Siamak Zarei-Ghanavati, Department of Ophthalmology, Khatam-al-Anbia Eye Hospital, Mashhad University of Medical Sciences (MUMS) Eye Research Center, Abootaleb Boulevard, Mashhad, 91959-65919, Iran; sizagh{at}yahoo.com, zareis{at}mums.ac.ir

Statistics from Altmetric.com

Recently, endothelial keratoplasty has been shown to offer a promising alternative to penetrating keratoplasty for the management of corneal endothelial failure. Melles et al1 introduced a new technique for transplantation of Descemet membrane (DM) through a self-sealing incision, which they referred to as Descemet membrane endothelial keratoplasty (DMEK). They obtained donor material by stripping DM with fine forceps. This is a challenging and time-consuming procedure with inevitable endothelial cell loss and possibility of DM tear. Herein, we describe a new technique to prepare donor tissue for DMEK, the reverse big-bubble technique.

Surgical technique

A corneoscleral button was placed endothelial side up on a donor Barron punch and fixed by grasping the outer scleral edge. The endothelial layer was stained with trypan blue. A 27-gauge bevel-up needle attached …

View Full Text

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.