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Antimicrobial peptides expression by ocular surface cells in response to Acanthamoeba castellanii: an in vitro study
  1. A M Otri1,
  2. I Mohammed1,
  3. A Abedin1,
  4. Z Cao2,
  5. A Hopkinson1,
  6. N Panjwani2,
  7. H S Dua1
  1. 1Division of Ophthalmology and Visual Sciences, University of Nottingham, Nottingham, UK
  2. 2New England Eye Centre, Tufts University School of Medicine, Boston, Massachusetts, USA
  1. Correspondence to Harminder S Dua, Division of Ophthalmology and Visual Sciences, B Floor, Eye ENT Centre, Queens Medical Centre, Nottingham University Hospitals NHS Trust, Derby Road, Nottingham NG7 2UH, UK; harminder.dua{at}nottingham.ac.uk

Abstract

Aims Antimicrobial peptides (AMPs) are natural effectors of the innate immune response. Much work has been done to study their response and effects on bacterial and viral infection. Little if any information is available in relation to protozoal infections. The aim of the study was to comprehensively study the gene expression of the ocular AMPs in human corneal limbal epithelial cells stimulated with Acanthamoeba castellanii (AC).

Methods Human corneal limbal epithelial cells were exposed to AC at different time points, up to 9 h, the genomic profile of the AMPs were analysed at these time point using real time PCR. corneal limbal epithelial cells not infected with AC were used as controls.

Results Seven of the eight studied AMPs showed statistically significant upregulation in gene expression. Human beta Defensin 3 (hBD3) showed a very significant 10-fold upregulation in the exposed cells and Ribonuclease-7 (RNase-7) showed a very early and consistent increase. Human beta Defensin 1 (hBD1) was the only downregulated AMP.

Conclusions The study data suggests a possible role of the AMPs in combating the amoebic infection at the ocular surface. Using AMPs singly or in combination is a promising avenue for further exploration in the treatment of the sight threatening Acanthamoeba keratitis.

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Footnotes

  • Funding Royal Blind Asylum and School, The Royal College of Surgeons, Edinburgh. Other Funders: NIH; Challenge grant from Research to Prevent Blindness to New England Eye Centre.

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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