Article Text

other Versions

PDF
Mycophenolic acid suppresses human pterygium and normal tenon fibroblast proliferation in vitro
  1. Radgonde Amer1,
  2. Liane Rabinowich1,
  3. Genia Maftsir1,
  4. Ilaria Puxeddu2,
  5. Francesca Levi-Schaffer2,
  6. Avraham Solomon1
  1. 1Department of Ophthalmology, Hadassah University Hospital, Jerusalem, Israel
  2. 2Department of Pharmacology, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel
  1. Correspondence to Dr Radgonde Amer, Department of Ophthalmology, Hadassah University Hospital, POB 12000, Jerusalem 91120, Israel; radgondeamer{at}yahoo.com

Abstract

Aims To investigate whether mycophenolic acid (MPA) exerts antifibrotic effects on pterygium fibroblasts (PFB) with and without stimulation with fibrogenic cytokines, and to compare the efficacy of MPA with mitomycin (MMC) and dexamethasone (DXM) on PFB and tenon fibroblasts (TFB).

Methods TFB and PFB were obtained from tissue explants during strabismus or pterygium surgery. Proliferation of subconfluent fibroblasts±basic fibroblast growth factor (bFGF) (10 ng/ml) was assessed by using the (3H) thymidine-incorporation assay. Cell cultures were incubated with MPA, MMC or DXM. Apoptosis was evaluated by quantifying Annexin V and propidium iodide positive cells with flow cytometry.

Results MPA showed a concentration-dependent inhibition of proliferation of PFB±bFGF as well as TFB±bFGF. The antiproliferative effect of MPA was comparable with that of MMC and DXM. Short exposure of PFB to MPA under profibrogenic conditions was significantly inhibitory. No apoptotic effect was found on TFB.

Conclusions MPA suppressed tenon and pterygium fibroblast proliferation in vitro under basal and profibrogenic conditions. It was comparable with MMC under long-term exposure, but MMC was more suppressive under short-term exposure. MPA may be safer than MMC due to a more specific mechanism of action and lack of cytotoxicity. Further investigation is warranted regarding MPA concentrations that will lead to a potent antiproliferative effect in vivo.

  • Mycophenolic acid
  • pterygium
  • ocular surface fibrosis
  • mitomycin-c
  • ocular surface
  • conjunctiva
  • cornea
  • Accepted 8 May 2010

Statistics from Altmetric.com

Footnotes

  • Competing interests None.

  • Patient consent Obtained.

  • Provenance and peer review Not commissioned; externally peer reviewed.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles

  • At a glance
    Harminder S Dua Arun D Singh