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We read with great interest the article by Shiraishi et al1 who have described a good technique for the laboratory diagnosis of Acanthamoeba cysts in corneal scrapings of patients with clinical features of Acanthamoeba keratitis using Fungiflora Y (FFY). We would like to share some of our comments related to this study.
Though described by the authors as a simple and fast technique, it calls for the use of expensive machinery such as the cryotome and expertise to take frozen sections. The availability of such equipment cannot be taken for granted even …