Article Text

other Versions

PDF
Evaluation of mouse experimental autoimmune uveoretinitis by spectral domain optical coherence tomography
  1. Kohzou Harimoto1,
  2. Masataka Ito2,
  3. Yoko Karasawa1,
  4. Yutaka Sakurai1,
  5. Masaru Takeuchi1
  1. 1Department of Ophthalmology, National Defense Medical College, Saitama, Tokorozawa, Japan
  2. 2Department of Developmental Anatomy and Regenerative Biology, National Defense Medical College, Saitama, Tokorozawa, Japan
  1. Correspondence to Professor Masaru Takeuchi, Department of Ophthalmology, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan; masatake{at}ndmc.ac.jp

Abstract

Aims To evaluate the efficacy of spectral domain optical coherence tomography (SD-OCT) in monitoring the development of mouse experimental autoimmune uveoretinitis (EAU) as an animal model of endogenous uveitis, and to develop an OCT-based grading system for EAU severity.

Methods C57BL/6 mice were immunised with human interphotoreceptor retinoid-binding protein (amino acid sequence 1–20) peptide and complete Freund's adjuvant to induce EAU. The development of EAU was monitored by SD-OCT serially throughout the disease course, and the images were graded from 1 to 4 and compared with the clinical and histopathological grades.

Results SD-OCT images depicted retinal lamella structures including the inner segment/outer segment (IS/OS) line in normal mice. Retinal structural changes were observed on SD-OCT images in mice that developed EAU clinically scored as grade 1 or higher, which precisely corresponded to the pathological findings. The SD-OCT images of EAU were graded as follows: grade 1, a few infiltrating cells in the vitreous and retina; grade 2, increased vitreous cells, retinal vasculitis, and granulomatous lesion; grade 3, cell infiltration into the whole retina, disappearance of IS/OS line, and destruction of the retinal layer structure; and grade 4, disappearance of the outer retina. The SD-OCT grade of EAU based on these criteria correlated significantly with both the clinical grade (R2=0.282, p<0.005) and histopathological grade (R2=0.846, p<0.0001).

Conclusions SD-OCT is useful for evaluating the development and severity of mouse EAU. The SD-OCT scoring system we developed accurately reflects clinical and histopathological changes.

  • Immunology

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.