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Blue light is a part of the visible light spectrum in the wavelength range of 390–490 nm. In modern society, light-emitting diode lamps and computer displays deliver much more blue light to the cornea than ever before. The retinal phototoxicity of blue light has been extensively investigated because of concerns about macular degeneration.1 ,2 The effects of blue light on the health of the ocular surface are less clear.3 ,4 Like skin,5 the ocular surface is directly exposed to visible blue light and this contributes to photophobia and ocular pain.6 We have carried out culture experiments to examine the phototoxicity of blue light in corneal cells.
A continuous-wave laser device equipped with an indium gallium nitride laser diode (RV-1000, Richo Optical Industry, Hanamaki, Japan) was used as a blue light source. Two rabbit corneal epithelial cell lines (SIRC and RC-1) were cultured in Dulbecco’s Modified Eagle Medium containing 10% fetal …