Article Text

other Versions

PDF
Proteomic surveillance of putative new autoantigens in thyroid orbitopathy
  1. Kai-Chun Cheng1,2,3,
  2. Chun-Tzu Hung4,
  3. Kai-Yuan Cheng5,
  4. Kuo-Jen Chen2,
  5. Wen-Chuan Wu3,6,
  6. Jau-Ling Suen1,
  7. Yu-Jen Wu7,
  8. Cheng-Hsien Chang1,2,3,6
  1. 1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
  2. 2Department of Ophthalmology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan
  3. 3Department of Ophthalmology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
  4. 4Department of Ophthalmology, Yuan's General Hospital, Kaohsiung, Taiwan
  5. 5Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
  6. 6Department of Ophthalmology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
  7. 7Department of Beauty Science, Meiho University, Pingtung, Taiwan
  1. Correspondence to Professor Cheng-Hsien Chang, Department of Ophthalmology, Kaohsiung Medical University Hospital, No. 100, Zihyou 1st Road, Sanmin District, Kaohsiung 807, Taiwan; hankorbit{at}hotmail.com

Abstract

Aims Thyroid orbitopathy (TO) is an autoimmune inflammatory disorder characterised by several ocular manifestations. Several autoantigens have been proposed to be involved in the pathogenesis of TO, but the autoantigen system and the mechanism of TO would be rather complex. In this study, an immunoproteomic method was used to survey novel autoantigens expressed in the orbital fat tissue of patients with TO.

Methods We used immunoproteomic, ELISA and immunohistochemical staining methods to survey novel autoantigens expressed in the orbital fat tissue of patients with TO.

Results Six protein spots showing high reactivity with the serum from the patients with TO were detected as candidate orbital autoantigens, and two of them (carbonic anhydrase 1 (CA1) and alcohol dehydrogenase 1B (ADH1B)) were further verified by ELISA and immunohistochemical staining. We found that CA1 and ADH1B could attribute target autoantigens in this autoimmune disease. We discovered anti-CA1 and anti-ADH1B antibody prevalence to be higher in patients with TO (68.57%/51.43%) or Graves’ disease (GD) (72%/48%) than in healthy controls respectively. Immunohistochemical staining study revealed the significantly enhanced expressions of CA1 and ADH1B in orbital fat of TO compared with that in healthy controls.

Conclusions We found that CA1 and ADH1B could attribute target autoantigens in this autoimmune disease. The high prevalence of these autoantibodies against CA1 and ADH1B in patients with TO and GD clarifies the potential clinical role for anti-CA1 and anti-ADH1B antibodies as biomarkers for GD and TO.

  • Immunology
  • Orbit

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Linked Articles

  • At a glance
    Keith Barton James Chodosh Jost Jonas