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Intravital imaging of the cellular dynamics of LysM-positive cells in a murine corneal suture model
  1. Mayumi Ueta1,2,
  2. Ayaka Koga2,
  3. Junichi Kikuta3,
  4. Keiko Yamada2,
  5. Sachi Kojima4,
  6. Katsuhiko Shinomiya2,
  7. Masaru Ishii3,
  8. Shigeru Kinoshita1
  1. 1Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  2. 2Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  3. 3Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Bioscience, Osaka University, Osaka, Japan
  4. 4Department of Ophthalmology, Faculty of Life Science, Kumamoto University, Kumamoto, Japan
  1. Correspondence to Dr Mayumi Ueta, Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Hirokoji, Kawaramachi, Kamigyo-ku, Kyoto 602-0841, Japan; mueta{at}koto.kpu-m.ac.jp

Abstract

Background Corneal suturing is a surgical procedure used in patients with corneal trauma or transplants. It was reported that endogenous neutrophils are brightly labelled in gene-targeted mice expressing enhanced green fluorescent protein (eGFP) under the control of the endogenous lysozyme M promoter (LysM-eGFP mice).

Methods We applied intravital imaging methods to analyse in vivo the dynamics of LysM-positive granulocytes (neutrophils) in LysM-eGFP mice with corneal sutures and examined their role in the elicitation of neutrophil infiltration.

Results We found that in the presuturing state, neutrophils strongly positive for LysM were located in the periphery of the corneal stromal layer; none were present in the centre of the cornea. After introducing a corneal suture, neutrophils accumulated in limbal vessels and then migrated to the corneal side and the conjunctival side, suggesting that they derived from limbal vessels. Thereafter they accumulated towards the central corneal area, arriving at the suture about 7 h after its placement. Although corneal sutures may elicit the continuous infiltration of neutrophils, their number was markedly decreased by day 1 after suture removal and continued to decrease thereafter.

Conclusions Our results showed that corneal sutures may elicit the continuous infiltration of neutrophils.

  • Cornea
  • Imaging
  • Inflammation

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