Article Text

PDF
Nerve terminals at the human corneoscleral limbus
  1. Mouhamed A Al-Aqaba,
  2. Fady S Anis,
  3. Imran Mohammed,
  4. Harminder S Dua
  1. Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, United Kingdom
  1. Correspondence to Professor Harminder S Dua, Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, United Kingdom; harminder.dua{at}nottingham.ac.uk

Abstract

Aims To demonstrate and characterise distinct subepithelial compact nerve endings (CNE) at the human corneoscleral limbus.

Methods Ten fresh human donor corneoscleral discs (mean age, 67 years) and 26 organ-cultured corneoscleral rims (mean age, 59 years) were studied. All samples were subjected to enzyme histochemical staining related to endogenous acetylcholinesterase present in nerve tissue and H&E staining. Whole-mount en face imaging with NanoZoomer digital pathology microscope and serial cross-section imaging with light microscope were undertaken.

Results Nerves entering the corneoscleral limbus and peripheral cornea terminate under the epithelium as enlarged multiloculated and multinucleated ovoid structures within a 2 mm zone. They are closely associated with the rete pegs of the limbal palisades and the limbal epithelial crypts, often located within characteristic stromal invaginations of these structures. Their numbers ranged from 70 to 300 per corneoscleral rim. The size ranged from 20 to 100 µm. They had one or more nerve connections and were interconnected to other similar endings and to the limbal nerve plexus.

Conclusion Human corneoscleral limbus demonstrates a population of nerve terminals resembling CNE with distinct morphological features. They are closely associated with the limbal stem cell niches, suggesting a potential contribution to the niche environment.

  • cornea
  • conjunctiva
  • anatomy
  • experimental– laboratory

Statistics from Altmetric.com

Footnotes

  • Contributors Concept and research design: MAAl-A and HSD. Data collection: MAAl-A and FSA. Data analysis and interpretation: MAAl-A, FSA, IM and HSD. Supervision: HSD. All authors participated in manuscript preparation.

  • Funding Elizabeth C. King Trust, Pittsburgh, PA, and the Philadelphia Eye Research Foundation.

  • Competing interests None declared.

  • Ethics approval The study was approved by the East Midlands Research Ethics Committee 2 (REC no 06/Q2403/46).

  • Provenance and peer review Not commissioned; externally peer reviewed.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.