Preliminary identification of the human macular pigment

doi:10.1016/0042-6989(85)90123-3

Vision Research

Volume 25, Issue 11, 1985, Pages 1531-1535

https://doi.org/10.1016/0042-6989(85)90123-3 Get rights and content

Abstract

The human macular pigment has been found to be composed of two chromatographically separable components, which are tentatively identified as lutein [(3R,3′R,6′R)-β,ε-Carotene-3,3′-diol] and zeaxanthin [(3R,3′R)-β,β-Carotene-3,3′-diol]. Chromatograms of retinal extracts, obtained by HPLC on three different stationary phases, were found to match those obtained with mixtures of lutein and zeaxanthin standards. Identical retention times were confirmed by coinjection of each of the isolated components with the appropriate standard. U.V.-visible spectra of the purified components were identical in all respects with those of lutein and zeaxanthin. Further support for our identification was obtained by the preparation and Chromatographie comparison of derivatives of the macular pigment and of the standards.

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View full text

Preliminary identification of the human macular pigment

Abstract

Vision Res.

Reversed-phase high-performance liquid chromatography of chlorophylls and carotenoids

Biochim. biophys. Acta

Biosynthesis of carotenoids

Visual pigments in human and monkey retinas

Nature, Lond.

On the structure of “Hydroxy-α-Carotene” from orange juice

Food Res.

Die Isolierung und quantitative Bestimmung der Carotenoide und Chlorophylle von Blättern, Algen und isolierten Chloroplasten mit Hilfe dünnschichtchromatographischer Methoden

Planta

Selective preparation of the lutein monomethyl ethers

Acta chem. scand.

Carotenoid pigments: their possible role in protecting against photooxidation in eyes and photoreceptor cells

Diet-related macular anomalies in monkeys

Invest. Ophthal. visual Sci.

The Retina