Rf values of fluorophores formed during incubation of vitamin A with liposomes and BSA
| Substrate mixture | Rf values of bands | ||
|---|---|---|---|
| Fresh | Incubated | ||
| pH 7 | |||
| LIP | 0.35(B),0.41(B),0.49(B),0.59(B) | 0.11(Y/G),0.19(Y),0.27(B/G),0.29(B/G), 0.32(B), 0.35(B),0.46(B),0.57(B),0.65(B),0.73(B) | |
| BSA | no visible bands | no visible bands | |
| LIP + BSA | 0.35(Y),0.48(B),0.57(Y) | 0.35(b),0.48(Y),0.57(B),0.86(Y) | |
| Retinal | 0.58–0.6(O)* | 0.6–0.65(O)*, 0.92(P)* | |
| Retinol | 0.625–0.675(Y) | 0.42–0.51(Y)*, 0.55–0.75(Y)* | |
| LIP + retinal | 0.35(B),0.46(Y),0.59(B) | 0.11(Y),0.24(Y),0.27(Y),0.38(O/Y), O.5(O),0.54(O),0.73(O),0.95(O),0.97(O) | |
| BSA + retinal | 0.33(Y),0.79(O) | 0.35(Y),0.56(P)*,0.85(O) | |
| LIP + BSA + retinal | 0.35(B),0.46(Y),0.59(B) | 0.11(Y),0.24(Y),0.27(Y),0.38(O/Y),O.5(O), 0.54(O),0.73(O),0.95(O),0.97(O) | |
| LIP + retinol | 0.35(B),0.46(Y),0.59(B) | 0.32(B),0.35(B),0.46(B),0.57(B),0.65(B),0.73(B) | |
| BSA + retinol | 0.36(B) | 0.43(Y), 0.59–0.73(Y),0.76(O)*+smear | |
| LIP + BSA + retinol | 0.35(B),0.48(Y),0.59(B) | 0.37(B),0.48(Y),0.57(B),0.86(Y) | |
Letters in parentheses indicate colour of bands (O = orange, B = blue, Y = yellow, P = purple). Rf = distance travelled (band/solvent front). *= non-fluorescent band; LIP = liposomes; BSA = bovine serume albumin.